Respon Dosis Vaksin Inaktif Chlamydosis

Proceedings of Temu Ilmiah VIII, 1999: 269
Pub. ISSN : 0918-7685

Pudjiatmoko

National Veterinary Drug Assay Laboratory , Gunungsindur, Bogor 162340

Nine 12-week-old specific-pathogen-free (SPF) cats were randomly divided into three groups. Groups A and B were vaccinated with an experimental vaccine derived from the Fe/145 strain of feline Chlamydia psittaci. Cats of group A were injected intramuscularly with vaccine containing 10^5.5ELD50 of Chlamydia and those of the group B were vaccinated with 10^4.5ELD50 of Chlamydia. Cats of group C were injected with suspension of sucrose-phosphate-glutamic acid and adjuvant as control. Vaccinations were performed twice. The second vaccination was done on 3 weeks post first vaccination. One week post second vaccination, cats were challenged intraocular with live feline C. psittaci B166 strain. Two cats of group A showed clinical signs of Chlamydiosis. All cats of the groups B and C showed the clinical signs. IgG antibody titer was determined by using microimmunofluorescence test. On the day of challenge, IgG antibody titers of cats of group A were 1:128 to 1:256, while those of group B were 1:64 to 1:128. The results indicate that cats should be injected with the vaccine containing Chlamydia more than 10^5.5ELD50 to give good protection against infection of Chlamydia.

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In vitro susceptibility of Chlamydia pecorum to macrolides, tetracyclines, quinolones and beta-lactam.

Microbiol Immunol. 1998; 42(1): 61-3.

Pudjiatmoko, Fukushi H, Ochiai Y, Yamaguchi T, and Hirai K.

Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University,

Yanagido, Japan.

The in vitro susceptibility of Chlamydia pecorum to two macrolides (clarithromycin and erythromycin), two tetracyclines (doxycycline and minocycline), two quinolones (ofloxacin and ciprofloxacin) and one beta-lactam (ampicillin) was determined. The MICs were 0.004 to 0.008 microg/ml for clarithromycin, 0.008 to 0.031 microg/ml for doxycycline and minocycline, 0.063 to 0.125 microg/ml for erythromycin, 0.25 to 0.5 microg/ml for ofloxacin and 0.25 to 1.0 microg/ml for ciprofloxacin. The MIC for ampicillin was greater than 1,024 microg/ml. The results show clarithromycin and doxycycline are the two most effective drugs against C. pecorum.

Rating: 5 Reviewer: Info Petani - ItemReviewed: In vitro susceptibility of Chlamydia pecorum to macrolides, tetracyclines, quinolones and beta-lactam. - 9756people
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Seroepidemiology of feline chlamydiosis by microimmunofluorescence assay with multiple strains as antigens.

Microbiol Immunol. 1996 ;40 (10):755-9

Pudjiatmoko, Hideto Fukushi, Yoshitsugu Ochiai, Tsuyoshi Yamaguchi and Katsuya Hirai

Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Yanagido 1-1, Gifu 50I-11, Japan

Abstract

The prevalence of anti-chlamydia antibodies was examined in 232 cat sera collected in 1985 and from 1993 to 1995 from laboratories and veterinary hospitals located in 11 prefectures of Japan. The antibodies were determined by an indirect microimmunofluorescence test using six strains of feline Chlamydia: one strain each of avian- and guinea pig-derived C. psittaci and one strain each of C.pecorum, C.pneumoniae and C.trachomatis. Positive rates of IgG antibodies to chlamydiae were 34.4% in 1985 and 16.5-21.4% from 1993 to 1995. Positive rates of IgM antibodies to chlamydiae were 8.2% in 1985 and 6.6-14.3% from 1993 to 1995. Variations in antibody reactivity to the different feline strains were observed. The results suggest the wide prevalence of chlamydial infection in cats in Japan, and antigenic diversity in the feline strains of C.psittaci.

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Diversity of feline Chlamydia psittaci revealed by random amplification of polymorphic DNA

Veterinary Microbiology
Volume 54, Issue 1, January 1997, Pages 73-83

Pudjiatmoko, Hideto Fukushi, Yoshitsugu Ochiai, Tsuyoshi Yamaguchi and Katsuya Hirai

Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Yanagido 1-1, Gifu 50I-11, Japan
Received 25 April 1996; accepted 5 August 1996. ; Available online 9 December 1997.

Abstract

DNA samples from C. psittaci including 6 strains of feline origin, 10 strains of avian origin, 1 strain of ovine origin and 1 strain of guinea pig origin were amplified each with three 10-nucleotide (nt) primers and four > 18-nt primers. Amplified products were separated by polyacrylamide gel electrophoresis. Eight patterns were recognized by random amplification of polymorphic DNA (RAPD) fingerprinting of C. psittaci: 2 patterns of feline origin, 5 patterns of avian origin and 1 pattern of guinea pig origin. DNA of feline or guinea pig origin was clearly distinguished from the other strains of C. psittaci by RAPD analysis, as shown by the absence of any common fragments in electrophoresis. The RAPD analysis indicated at least 2 types of feline C. psittaci. The RAPD typing is suggested as a convenient tool for molecular epidemiology of chlamydial infection.

Author Keywords: Chlamydia psittaci; Cat; Polymerase chain reaction; DNA amplification

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Phylogenetic analysis of the genus Chlamydia based on 16S rRNA gene sequences

International Journal of Systematic Bacteriology, Vol 47, 425-431

Pudjiatmoko, H Fukushi, Y Ochiai, T Yamaguchi and K Hirai
Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.

The phylogenetic relationships among Chlamydia spp. were investigated by comparing 16S rRNA gene sequences. In this analysis we used 14 strains of Chlamydia psittaci, including seven feline isolates, two avian isolates, two human isolates, one bovine isolates, one ovine isolate, and one koala isolate; and nine strains of Chlamydia trachomatis, including six human isolates, two swine isolates, and one mouse isolate. A phylogenetic analysis of the 16S rRNA gene sequences of these organisms and seven previously published sequences revealed eight genetic groups which formed two clusters. The first cluster was composed of C. pecorum, Chlamydia pneumoniae, and C. psittaci and included three genetic groups (one group containing avian, human, and ovine strains, one group containing feline strains, and one group containing guinea pig strains). The second cluster was composed of C. trachomatis and also included three genetic groups (one group containing human strains, one group containing swine isolates, and one group containing rodent strains). The strains in each genetic group exhibited similar genetic distances. The results of the phylogenetic analysis agreed with the results of previous genomic DNA, ompA gene allele, and biotyping studies. Therefore, the genetic groups based on genetic distances may be considered a criterion for species identification.

Rating: 5 Reviewer: Info Petani - ItemReviewed: Phylogenetic analysis of the genus Chlamydia based on 16S rRNA gene sequences - 9756people
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